Progenitor Cell Homing in the Postischemic Myocardium: Just an Unmotivated Pitstop in the Microcirculation?
Identifieur interne : 000235 ( Main/Exploration ); précédent : 000234; suivant : 000236Progenitor Cell Homing in the Postischemic Myocardium: Just an Unmotivated Pitstop in the Microcirculation?
Auteurs : Fabio Tuche [Allemagne, Brésil] ; Michael D. Menger [Allemagne] ; Christina Körbel [Allemagne] ; Ruth M. Nickels [Allemagne] ; Eliete Bouskela [Brésil] ; René Schramm [Allemagne]Source :
- Microcirculation [ 1073-9688 ] ; 2012-11.
English descriptors
- Teeft :
- Adhesion, Adhesion molecules, Antegrade, Aortic arch, Apoe, Arteriole, Atherosclerosis, Atherosclerotic, Atherosclerotic apoe, Bmcs, Cardiac, Cardiac syngrafts, Cell therapy, Chavakis, Clone, Contrast enhancement, Coronary arterioles, Coronary capillaries, Coronary microcirculation, Dimmeler, Direct antegrade intracoronary injection, Endothelial, Endothelial progenitor cells, Epicardial surface, Fetal calf serum, Functional involvement, Generalized atherosclerosis, Graft, Homing, Image analyses software, Infarcted heart, Infarction, Intracoronary, Intravital, Intravital microscopy, Ischemia, Ischemic, Ischemic tissues, John wiley sons, Leukocyte, Leukocyte recruitment, Microcirculation, Microcirculatory parameters, Microvascular, Mutant animals, Myocardial capillaries, Myocardium, Nutritive, Nutritive capillaries, Perfusion, Polyethylene catheter, Postcapillary, Postcapillary venules, Postischemic, Postischemic myocardium, Progenitor, Progenitor cell, Progenitor cell characteristics, Progenitor cells, Pulmonary trunc, Quantitative analyses, Reperfusion, Schramm, Sequestration, Subepicardial, Subepicardial microcirculation, Surface expression, Syngeneic, Syngeneic bmcs, Syngraft, Syngrafts, Transected distally, Transplantation, Uorescently, Venule.
Abstract
We developed a model for direct assessment of BMC sequestration in the postischemic murine myocardium after direct antegrade intracoronary injection.
Modified syngeneic heterotopic heart transplantation was used as a basic model for global myocardial I/R injury in a total of n = 29 animals. IVM was employed to analyze the right ventricular subepicardial coronary microcirculation and for tracking fluorescently labeled BMCs.
IVM allowed monitoring all segments of the coronary microcirculation including feeding arterioles, nutritive capillaries, and postcapillary venules. WI and generalized atherosclerosis induced profound reperfusion failure, particularly in nutritive myocardial capillaries. BMCs were found to exclusively sequester in myocardial capillaries, but not in coronary arterioles or postcapillary venules. The sequestration of BMCs in coronary capillaries occurred independent of WI, generalized atherosclerosis, or adhesion molecule function.
This is the first study allowing direct assessment of BMC homing to the postischemic myocardium. Heterotopic heart transplantation and IVM are proper means to study the myocardial sequestration of BMCs after direct antegrade intracoronary injection in vivo. We show for the first time that intracoronarily injected BMCs sequester exclusively in nutritive myocardial capillaries.
Url:
DOI: 10.1111/j.1549-8719.2012.00212.x
Affiliations:
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type="eISSN">1549-8719</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2012-11">2012-11</date><biblScope unit="volume">19</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="739">739</biblScope><biblScope unit="page" to="748">748</biblScope></imprint><idno type="ISSN">1073-9688</idno></series><idno type="istex">CEB0C50E81283B9965FB6A18CA7F2766B1DE5814</idno><idno type="DOI">10.1111/j.1549-8719.2012.00212.x</idno><idno type="ArticleID">MICC212</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1073-9688</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adhesion</term><term>Adhesion molecules</term><term>Antegrade</term><term>Aortic arch</term><term>Apoe</term><term>Arteriole</term><term>Atherosclerosis</term><term>Atherosclerotic</term><term>Atherosclerotic apoe</term><term>Bmcs</term><term>Cardiac</term><term>Cardiac syngrafts</term><term>Cell therapy</term><term>Chavakis</term><term>Clone</term><term>Contrast enhancement</term><term>Coronary arterioles</term><term>Coronary capillaries</term><term>Coronary microcirculation</term><term>Dimmeler</term><term>Direct antegrade intracoronary injection</term><term>Endothelial</term><term>Endothelial progenitor cells</term><term>Epicardial surface</term><term>Fetal calf serum</term><term>Functional involvement</term><term>Generalized atherosclerosis</term><term>Graft</term><term>Homing</term><term>Image analyses software</term><term>Infarcted heart</term><term>Infarction</term><term>Intracoronary</term><term>Intravital</term><term>Intravital microscopy</term><term>Ischemia</term><term>Ischemic</term><term>Ischemic tissues</term><term>John wiley sons</term><term>Leukocyte</term><term>Leukocyte recruitment</term><term>Microcirculation</term><term>Microcirculatory parameters</term><term>Microvascular</term><term>Mutant animals</term><term>Myocardial capillaries</term><term>Myocardium</term><term>Nutritive</term><term>Nutritive capillaries</term><term>Perfusion</term><term>Polyethylene catheter</term><term>Postcapillary</term><term>Postcapillary venules</term><term>Postischemic</term><term>Postischemic myocardium</term><term>Progenitor</term><term>Progenitor cell</term><term>Progenitor cell characteristics</term><term>Progenitor cells</term><term>Pulmonary trunc</term><term>Quantitative analyses</term><term>Reperfusion</term><term>Schramm</term><term>Sequestration</term><term>Subepicardial</term><term>Subepicardial microcirculation</term><term>Surface expression</term><term>Syngeneic</term><term>Syngeneic bmcs</term><term>Syngraft</term><term>Syngrafts</term><term>Transected distally</term><term>Transplantation</term><term>Uorescently</term><term>Venule</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">We developed a model for direct assessment of BMC sequestration in the postischemic murine myocardium after direct antegrade intracoronary injection.</div><div type="abstract">Modified syngeneic heterotopic heart transplantation was used as a basic model for global myocardial I/R injury in a total of n = 29 animals. IVM was employed to analyze the right ventricular subepicardial coronary microcirculation and for tracking fluorescently labeled BMCs.</div><div type="abstract">IVM allowed monitoring all segments of the coronary microcirculation including feeding arterioles, nutritive capillaries, and postcapillary venules. WI and generalized atherosclerosis induced profound reperfusion failure, particularly in nutritive myocardial capillaries. BMCs were found to exclusively sequester in myocardial capillaries, but not in coronary arterioles or postcapillary venules. The sequestration of BMCs in coronary capillaries occurred independent of WI, generalized atherosclerosis, or adhesion molecule function.</div><div type="abstract">This is the first study allowing direct assessment of BMC homing to the postischemic myocardium. Heterotopic heart transplantation and IVM are proper means to study the myocardial sequestration of BMCs after direct antegrade intracoronary injection in vivo. We show for the first time that intracoronarily injected BMCs sequester exclusively in nutritive myocardial capillaries.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Brésil</li></country><region><li>Bavière</li><li>District de Haute-Bavière</li><li>État de Rio de Janeiro</li></region><settlement><li>Munich</li><li>Rio de Janeiro</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Tuche, Fabio" sort="Tuche, Fabio" uniqKey="Tuche F" first="Fabio" last="Tuche">Fabio Tuche</name></noRegion><name sortKey="Korbel, Christina" sort="Korbel, Christina" uniqKey="Korbel C" first="Christina" last="Körbel">Christina Körbel</name><name sortKey="Menger, Michael D" sort="Menger, Michael D" uniqKey="Menger M" first="Michael D." last="Menger">Michael D. Menger</name><name sortKey="Nickels, Ruth M" sort="Nickels, Ruth M" uniqKey="Nickels R" first="Ruth M." last="Nickels">Ruth M. Nickels</name><name sortKey="Schramm, Rene" sort="Schramm, Rene" uniqKey="Schramm R" first="René" last="Schramm">René Schramm</name><name sortKey="Schramm, Rene" sort="Schramm, Rene" uniqKey="Schramm R" first="René" last="Schramm">René Schramm</name><name sortKey="Schramm, Rene" sort="Schramm, Rene" uniqKey="Schramm R" first="René" last="Schramm">René Schramm</name></country><country name="Brésil"><region name="État de Rio de Janeiro"><name sortKey="Tuche, Fabio" sort="Tuche, Fabio" uniqKey="Tuche F" first="Fabio" last="Tuche">Fabio Tuche</name></region><name sortKey="Bouskela, Eliete" sort="Bouskela, Eliete" uniqKey="Bouskela E" first="Eliete" last="Bouskela">Eliete Bouskela</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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